Compact oleic acid in HAMLET

Who Is Mr. HAMLET? Interaction of Human R-Lactalbumin with Monomeric Oleic Acid

A specific state of the human milk Ca2+ binding protein R-lactalbumin (hLA) complexed with oleic acid (OA) prepared using an OA-pretreated ion-exchange column (HAMLET) triggers several cell death pathways in various tumor cells. The possibility of preparing a hLA-OA complex with structural and cytotoxic properties similar to those of the HAMLET but under solution conditions has been explored. T...

Structural Origin of ELOA Toxicity – Implication for HAMLET-Type Protein Complexes with Oleic Acid

Oleic acid is a key cytotoxic component of HAMLET-like complexes.

HAMLET is a complex of α-lactalbumin (α-LA) with oleic acid (OA) that selectively kills tumor cells and Streptococcus pneumoniae. To assess the contribution of the proteinaceous component to cytotoxicity of HAMLET, OA complexes with proteins structurally and functionally distinct from α-LA were prepared. Similar to HAMLET, the OA complexes with bovine β-lactoglobulin (bLG) and pike parvalbumin ...

Applications of Site-Specific Labeling to Study HAMLET, a Tumoricidal Complex of α-Lactalbumin and Oleic Acid

BACKGROUND Alpha-lactalbumin (α-LA) is a calcium-bound mammary gland-specific protein that is found in milk. This protein is a modulator of β1,4-galactosyltransferase enzyme, changing its acceptor specificity from N-acetyl-glucosamine to glucose, to produce lactose, milk's main carbohydrate. When calcium is removed from α-LA, it adopts a molten globule form, and this form, interestingly, when c...

Conversion of oleic acid to linoleic acid.

Reports from this laboratory have described the formation of palmitoleic and oleic acids from their saturated counterparts by enzyme systems of yeast (2, 3). For introducing a double bond into the 9,lO position of the aliphatic chain, the desaturating enzymes, acting on the coenzyme A derivatives of the fatty acids, require reduced triphosphopyridine nucleotide, and molecular oxygen as hydrogen...